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KMID : 0613820100200060955
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Journal of Life Science
2010 Volume.20 No. 6 p.955 ~ p.959
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Multiplex PCR-aided Differential Diagnosis of Taeniid Species
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Lee Hye-Jung
Kwak Sahng-June
Seo Min
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Abstract
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Differential diagnosis of the taeniid proglottids between Taenia asiatica and T. sagniata is a daunting task due to their close morphological similarity. However, to correctly diagnose them on time is important in managing infected patients, as well as for reducing serious complications such as cysticercosis. Currently, DNA-based methods for the dissection of genomic information of parasites are being employed to make accurate and rapid diagnoses in the field of parasitology. In this study, multiplex PCR was established and exploited to identify exact species of taeniid adult worms recovered from Korean people. To discriminate one from the other other, primers-Ta4978F, Ts5058F, Tso7421F, and Rev7915- were used for the multiplex PCR, which provided swift and precise identification of the taeniid worms being observed. Also,having instituted PCR methodology, we ascertained that easiness would be achieved to reassess and re-evaluate Korean endemic data on human taeniid cestodes.
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KEYWORD
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T. asiatica, T. saginata, T. solium, multiplex PCR
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